Journal: Inflammation
Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia
doi: 10.1007/s10753-026-02476-9
Figure Lengend Snippet: Hyperoxia induces the increase in glycolysis levels in the lungs of BPD mice. a : Detect the lactate levels in the BALF of N7, H7, N14 and H14 groups. b : The mRNA expression level of PKM2 quantified by RT-qPCR. c : The activity of HK in the lung tissue of N7, H7, N14 and H14 groups. d : The protein levels of PKM2 in the lung tissue of N7, H7, N14 and H14 groups were detected by WB. e : Representative images of immunofluorescence after staining with anti-CD86 antibody (green) and anti-PKM2 antibody (red) at 14 days. Nuclei were stained by DAPI (blue). f : The relative fluorescence intensity of PKM2 in the lungs of each group at 14 days. Data are shown as means ± SD ( n = 3). * P < 0.05, ** P <0.01, *** P<0.001
Article Snippet: After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan).
Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Immunofluorescence, Staining, Fluorescence